Fig. 3.

Deletion of Ptpn11 causes cell death at the diencephalic-mesencephalic junction but not in the posterior part of the mesencephalon. (A-D) Immunofluorescence for Pax6 and activated Casp3 on sagittal sections of E9.5 embryos. B′ is Casp3-channel image of B with dashed lines outlining the neural tube; arrowheads indicate the isthmus; boxed areas are enlarged in C and D. (E) Quantification of Casp3⁺ cells at the diencephalic-mesencephalic junction on medial (M), intermediate (I) and lateral (L) sections of the neural tube (mean±s.e.m.) of wild-type (WT) and Ptpn11-cKO embryos. Two-tailed unpaired t-test with Welch's correction, n=4 embryos per group. (F,G) Immunofluorescence for Casp3 on sagittal sections. The arrows in B′-G indicate Casp3⁺ cells.