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. 2016 Oct 15;143(20):3700–3710. doi: 10.1242/dev.135756

Fig. 4.

Fig. 4.

Contribution of Hes1+ and Wt1+ progenitor cells to the Gli1+ interstitial cell population in the fetal testis. (A-C) Lineage-tracing analysis of Hes1+ progenitor cells in the Hes1-CreERT2; Rosa-LSL-tdTomato; Gli1-lacZ embryos was induced by tamoxifen (TM) administration at E10.5 and E11.5. The samples were analyzed at E14.5 by fluorescence immunohistochemistry for Hes1-td (red; tdTomato-labeled fetal testis-derived Hes1+ cells), β-galactosidase (green for Gli1+ cells). (D-G) Lineage labeling of Wt1+ progenitor cells in the Wt1-CreERT2; Rosa-LSL-tdTomato; Gli1-lacZ embryos was induced by TM administration at E10.5. The samples were analyzed at E13.5 by fluorescence immunohistochemistry for Wt1-td (red; tdTomato-labeled fetal testis-derived Wt1+ cells), β-galactosidase (green for Gli1+ cells) and AMH (gray for Sertoli cells). The insets are higher magnification of the boxed areas. Scale bars: 25 μm.