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Myocardin is derepressed in ectopic smooth muscle. (A,B) In situ hybridization for myocardin (Myocd) reveals overlapping expression with SM22α and SMA in ectopic smooth muscle in Ezh2mesoderm-KO lungs (arrowheads, dashed lines) at E14.5 (A) and E18.5 (B). (C) ChIP-qPCR for H3K27me3 in isolated mesoderm at E18.5. The Myocd promoter is enriched relative to the non-repressed Actb locus and a nearby gene desert. Error bars indicate s.e.m. Scale bars: 50 µm in A; 100 µm in B.
