Fig. 4.

No apparent defects in proliferation and neurogenesis of the arcuate neurons in Isl1^cko mice. (A,B) Immunohistochemistry with anti-BrdU antibody (A) and ISH for Mash1 (B) were performed on the ARC of control and Isl1^cko mice at E12.5. BrdU was intraperitoneally injected to pregnant females 2 h before euthanasia. (C) ISH for Gad1, Gsx1 and Hmx2 on the ARC of control and Isl1^cko mice at E14.5 or E18.5. Signal intensity for Gad1 was quantified using ImageJ. (D) ISH with Gad1 performed on the ARC of control and Isl1^cko mice at P21. Dashed lines indicate the boundary of the ARC. Signal intensity for Gad1 was quantified using ImageJ. Double arrows indicate area expressing Gad1. (E) Immunohistochemistry with anti-Sox2 antibody performed on the ARC of control and Isl1^cko mice at P28. (F) Immunohistochemistry with anti-NeuN antibody was performed on the ARC of control and Isl1^cko mice at E16.5 and P15. The number of NeuN⁺ cells was quantified using ImageJ. Three controls and four mutants were used for all experiments. Scale bars: 100 μm. **P<0.01; Student's t-test. Error bars indicate s.e.m.