Fig. 4.

Knockdown of TNPO1 but not of importin-β1 reduces the ciliary targeting of fibrocystin. (A) TNPO1 and importin-β1 are specifically knocked down by the relevant siRNAs in RPE1 cells. GL2 siRNA is a negative control. (B,C) RPE1 cells were subjected to knockdown by siRNAs targeting GL2, importin-β1 and TNPO1. Cells were subsequently transfected to express f-CTS–GFP. Images and CPIR values were acquired after induction of ciliogenesis. In each image, the cilium of interest is enlarged and boxed at the upper right corner. Data in C were from n=75 cells. Error bars are s.e.m. The mean value is indicated at the top of each column. P-values (t-test) of selected pairs are denoted.