Fig. 2.
APC15 depletion induces a strong mitotic arrest in ΔUBE2C cells. (A,B) The indicated cell lines were treated with siRNAs targeting luciferase as a control, APC15 or p31comet and filmed as they progressed through mitosis. Each circle represents a single cell analyzed and the red line indicates the median, which is also stated in minutes above each condition. Red circles represents cells that were still arrested when recording ended. Representative stills on the right. Scale bar in stills: 10 μm. ns, non-significant; **P≤0.01, ****P≤0.0001 by Mann–Whitney test. For A, at least 121 cells per condition were analyzed from two independent experiments. (B) Representative experiment with at least 48 cells analyzed per condition. (C) Western blot analysis of lysates from the indicated cell lines showing the levels of APC15 and p31comet. The depletion efficiencies in the different conditions were: WT, 55% p31 and 51% APC15; ΔUBE2C, 64% p31 and 44% APC15; ΔUBE2S, 63% p31 and 57% APC15 when normalized to luciferase and setting control depletion to one.