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. 2016 Oct 15;5(10):1351–1361. doi: 10.1242/bio.019588

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© 2016. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 2. — The interaction with Filamin protects Refilins from degradation. (A) Sub-confluent FLNA-deficient M2 cells (lanes 1-3) and A7 cells (lanes 4-6) were not transfected (lanes 1,4) or transfected with Myc-tagged RefilinA (lanes 2,5) or RefilinB (lanes 3,6). Total cell extracts were analysed by western blot with anti-Myc and anti-FLNA antibodies. (B) M2 cells (left panel) and A7 cells (right panel) transfected with Myc-tagged RefilinA were fixed and stained with anti-Myc antibodies. (C) Sub-confluent FLNA-deficient M2 cells co-transfected with 19-22 FLNA-HA and either Myc-tagged control protein (Miret) (lane 1), RefilinA-ΔBD₁ to -ΔBD₄ mutants (lanes 2-5) or wild-type RefilinA (lane 6) were immunoprecipitated with rat anti-HA antibody. Total cell extracts (Total) and immunoprecipitates (IP-HA) were analysed by western blot using mouse anti-HA and mouse anti-Myc. In A and C, asterisks indicate to a non-specific band.