Fig. 2.

Absence of synthetic lethality between Polθ and candidate genes. (A,B) Potential hits were transfected in low throughput and cell viability was assessed by MTS test at 72 h (A) and 96 h (B) post-transfection. (C) RKO cells were transfected with siRNA against Polθ, and then seeded after 24 h incubation in a 96-well plate prior to transfection with the indicated siRNAs. Cell viability was assessed 96 h later by MTS test. Error bars represent standard deviation from three independent experiments.