Fig. 2.
tRNAiMet-driven cell migration is dependent on translation initiation ternary complex formation. iMEFs stably expressing tRNAiMet or vector control (Vector) were treated with salubrinal (75 μM) or vehicle control (DMSO) for 2 h (A). Alternatively these cells were transfected with a vector encoding the phosphatase, GADD34 or mock control (B). iMEF migration speed was then determined as for Fig. 1A. The trackplots indicate representative migration trajectories of these cells over a 17 h period. The western blots indicate the influence of salubrinal and GADD34 overexpression on levels of phosphorylated eIF2α. Data are represented as box and whisker plots (whiskers: 10-90 percentile); n=3 independent experiments; ****P<0.0001; ns, not significant; Mann–Whitney test. Scale bar: 100 μm.