Fig. 4.
Membrane-bound uncleavable Slit rescues the pattern of LT muscles in amon mutants. Upper panel indicates the knocked-in slit constructs. (A-E) Representative images of the LT muscles in WT (A), amon (B), knocked-in uncleavable slit (C), knocked-in uncleavable slit with CD8 (D), and a recombinant carrying knocked-in uncleavable Slit with amon mutant allele (E). All muscles are labeled with anti Tropomyosin (Tm). Bar in A represents 10 µm, indicating the magnification in all panels. (F) Quantification of the ration DLT3/DS, indicating a significant difference between slit-UC-CD8;amon and amon alone. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots; crosses represent sample means; bars indicate 83% confidence intervals of the means; data points are plotted as open circles. Gray and white represent the different experiments.