Fig. 4.

The major mRNA decay systems have similar activities in the wild-type and edc3Δ lsm4ΔC strains. (A) Deadenylation assay of transcribed MFA2 3′UTR with 50 3′ adenosines. Equal concentrations of purified TAP tagged Ccr4 from wild-type and edc3Δlsm4ΔC cells were used. Error=s.d.; n=3 biological replicates. (B) Decapping assay of m7G cap labeled mRNA. Equal concentrations of purified TAP tagged Dcp1 from wild-type and edc3Δlsm4ΔC cells were used. Error=s.d.; n=3 biological replicates. (C) Growth assay of yeast with a plasmid containing a non-stop variant of the HIS3 gene in strains deleted for the HIS3 gene as well as the gene indicated. Strains grown on synthetic complete plates lacking uracil or histidine respectively for both three and five days.