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. 2016 Aug 19;5(10):1388–1399. doi: 10.1242/bio.020487

Fig. 5.

Fig. 5.

Decay factor protein abundance, Dcp2 localization and mRNA levels in wild-type and edc3Δ lsm4ΔC mutant strains. (A) Quantification of C-terminal TAP tagged mRNA decay factors protein levels in wild-type and edc3Δ lsm4ΔC strains. Cells grown in either glucose or galactose containing media. Loading normalized to the Dpm1 protein abundance. (B) Wild-type and mutant strains depicting Dcp2-GFP intensity in unstressed live cells. To the right is the DAPI staining. (C) Percentage of Dcp2-GFP that is cytoplasmic as determined from sum-projected Z-stacks, masked and intensity within the cytoplasm, box showing quantification of cytoplasmic Dcp2-GFP (line, median; box 25th and 75th percentiles; whiskers, 10th and 90th percentiles, n=50 biological replicates). (D) The relative full-length mRNA was determined for MFA2 and PGK1 in the strains indicated as compared to the SCR1 loading control. The bars represent mean percentage of the indicated mRNA in the strain normalized to the WT background. Error bars indicate standard deviation, n=3 biological replicates. Significance determined in comparison to wild type by two-tailed t-test.