FIGURE 4.

Mycobacterial PASTA4 orthologs contain unusual conserved tryptophans. Sequence logo representations of Hidden Markov Models of a typical PASTA domain (Pfam PF03793.15) and of the four Mycobacterial PASTA domains. The mycobacterial logos were derived from an alignment of 72 unique full-length sequences of PknB orthologs. Residues in PASTA4 that were tested by alanine substitution and found essential are marked with red stars. N601A substitution produced a functional allele and is marked with a green star.