TABLE 2.
Transformation efficiency of KanR pknB plasmids into the M. tuberculosis strain carrying ΔpknB::hyg+pknB_WT at attBL5-Zeo
| Plasmid | pknB variant | Transformation efficiency/μg DNA (± S.D.) n = 3 |
|---|---|---|
| pKP570 | MCK-pknB_WT | 5.03 ± 0.5 × 104 |
| pKP571 | MCK-pknB_ΔPASTA1 | 0 |
| pKP572 | MCK-pknB_ΔPASTA2 | 0 |
| pKP573 | MCK-pknB_ΔPASTA3 | 0 |
| pKP574 | MCK-pknB_ΔPASTA4 | 0 |
| pKP575 | MCK-pknB_W571A | 0 |
| pKP576 | MCK-pknB_K589A | 0 |
| pKP577 | MCK-pknB_N601A | 3.41 ± 0.3 × 104 |
| pKP578 | MCK-pknB_F624A | 0 |
| pMV306 | L5 integrating vector | 0 |
| pMV261 | Replicating plasmid | 1.87 ± 0.2 × 105 |
| pTTP1A | Tweety Integrating plasmid | 6.50 ± 0.5 × 104 |