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The rpoEP4 promoter is regulated positively by RpoS and responds to its modulators. Cultures of SR18874 carrying the rpoEP4 promoter fusion, its ΔrpoS or ΔrssB derivatives, and derivative carrying the cloned pcnB gene were grown in LB medium at 30 °C either in the presence of 50 μm IPTG for strain with plasmid or without IPTG for plasmid-free derivatives. Samples from four independent cultures at different growth intervals in each case were analyzed for the β-galactosidase activity.
