TABLE 1.
Impact of non-polar deletion mutations or overexpression of various regulators that alter the activity of the rpoEP1-P5-lacZ transcriptional fusion
Values correspond to average of four independent samples from cultures grown in LB or in M9 minimal medium at A600 0.2 to 0.4.
| Function | Effect | Specificity | |
|---|---|---|---|
| Genotype | |||
| Wild-type SR7917 (rpoEP1-P5-lacZ) | 393 ± 26 | ||
| ΔwaaC | Heptosyltransferase I | 2630 ± 168 | rpoEP3 in Rcs-dependent manner, also induces the EσE-regulated rpoEP6 promoter that is only partially dependent on the Rcs system |
| ΔwaaF | Heptosyltransferase II | 1052 ± 65 | rpoEP3 |
| ΔwaaG | LPS glucosyltransferase I | 837 ± 46 | rpoEP3 |
| ΔwaaP | LPS core HepI kinase | 894 ± 51 | rpoEP3 |
| ΔrfaH | Transcriptional anti-terminator | 1505 ± 93 | rpoEP3 |
| Δcya | Adenylate cyclase | 230 ± 16 | rpoEP3 |
| Δcrp | Transcriptional dual regulator | 215 ± 18 | rpoEP3 |
| Δ(ecfLM) | RpoE regulon member, DedA family member | 870 ± 85 | rpoEP3 |
| ΔrssB | Regulator of RpoS | 531 ± 37 | rpoEP4 |
| ΔarcA | Transcriptional dual regulator | 831 ± 65 | rpoEP4 |
| ΔarcB | Sensory histidine kinase | 1120 ± 90 | rpoEP4 |
| ΔrpoS | RpoS σ factor | 193 ± 14 | rpoEP4 |
| ΔrpoN | RpoN σ factor | 302 ± 25 | rpoEP2, lack of RpoN in ΔwaaC, reduces rpoEP2 induction that is QseF-dependent |
| Δ(rpoN waaC) | 1290 ± 90 | ||
| ΔntrC | Transcriptional dual regulator | 312 ± 27 | rpoEP2 |
| Multicopy expression that alters the transcription | |||
| qseG | Part of qseE/F operon | 6920 ± 335 | rpoEP2 |
| yhdV and six other genes encoding lipoproteins | Lipoprotein | 1063 ± 68 | rpoEP3 (Rcs-dependent) |
| rirA | sRNA | 786 ± 52 | rpoEP3 |
| pcnB | Poly(A) polymerase I | 540 ± 38 | rpoEP4 |
| fliZ | DNA-binding transcriptional regulator | 249 ± 21 | rpoEP4 |