TABLE 2.
Supplementation of growth medium that alter the activity of the rpoEP1-P5-lacZ transcriptional fusion using strain SR7917
Cultures were grown in either LB medium adjusted to an A600 0.05, followed by challenge with specific reagent as indicated, or in nitrogen-limiting minimal medium containing 3 mm ammonium chloride as the nitrogen source. Values correspond to an average of four independent samples after a 30–60-min shift.
| Supplementation of growth medium that alter the transcription activity | ||
|---|---|---|
| Sucrose (0.25 m) | 1410 ± 110 | rpoEP4 |
| Glucose (0.5%) | 219 ± 15 | rpoEP3 |
| 25 mm Ammonium metavanadate | 1540 ± 90 | rpoEP2/P3 |
| 0.25 μg of Polymyxin B (A600 0.05) | 1503 ± 77 | rpoEP3 |
| 0.25 μg of Polymyxin B (A600 0.4) | 410 ± 29 | No induction |
| 3 mm Ammonium chloride | 575 ± 37 | rpoEP2 |