TABLE 1.

Steady-state rates of wild-type and S1179D eNOS full-length proteins

Rates were measured at 25 and 10 °C as described under “Experimental Procedures.” Values represent the mean ± S.D. of three independent measurements with two preparations of each enzyme.

Protein	NO Synthesis	NADPH oxidation
		+CaM	−CaM
	min−1
Activities at 25 °C
WT eNOS FL	10.5 ± 1	42 ± 2	6 ± 1
S1179D eNOS FL	22 ± 2	68 ± 3	11 ± 1
Activities at 10 °C
WT eNOS FL	6.5 ± 0.6	19 ± 2	4 ± 1
S1179D eNOS FL	12 ± 1	42 ± 3	6 ± 1