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Summary of results from reactions mixing eNOSr enzymes with excess cytochrome c
An excess of cytochrome c was mixed with fully reduced proteins in a stopped-flow instrument at 10 °C as described under “Experimental Procedures.”
| Protein | Condition | Time (first turnover) | Fraction open | Fraction closed | Keq | Steady-state electron flux |
|---|---|---|---|---|---|---|
| min−1 | ||||||
| WT eNOSr | −CaM | 1.85 s | 0.13 | 0.87 | 0.15 | 27.6 |
| +CaM | 110 ms | 0.60 | 0.40 | 1.5 | 186 | |
| S1179D eNOSr | −CaM | 134 ms | 0.60 | 0.40 | 1.5 | 129 |
| +CaM | 70 ms | 0.71 | 0.29 | 2.4 | 228 |
