TABLE 4.
Kinetic parameters derived from fitting the experimental traces according to the four-state kinetic model
| Protein | Condition | Measured Keq | Best fit k1 = k3 | Best fit k−1 = k−3 | Best fit k2 | Lower boundary value k1 or k3 | Lower boundary value k−1 or k−3 | Lower boundary value k2 | Fitted Steady state electron flux (experimental value) | Fitted Time required for first turnover (experimental value) |
|---|---|---|---|---|---|---|---|---|---|---|
| s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | s−1 | ||||
| WT eNOSr | −CaM | 0.15 | 4.2 | 0.63 | 5 | 3.2 | 0.55 | 0.8 | 0.46 (0.46) | 1.85 (1.85) s |
| +CaM | 1.5 | 8.8 | 13.2 | 17 | 5.0 | 7.7 | 9.9 | 3.0 (3.1) | 110 (110) ms | |
| S1179D eNOSr | −CaM | 1.5 | 8 | 12 | 9 | 3.4 | 5.3 | 5.1 | 2.15 (2.2) | 135 (134) ms |
| +CaM | 2.4 | 8.5 | 20.4 | 32.4 | 5.2 | 12.7 | 13 | 3.8 (3.7) | 70 (70) ms |