FIGURE 5.

Specific energy transfer between adjacent GCPs within γTuRCs at centrosomal Nlp assemblies. A, localization of γ-tubulin and GCPs in U2OS cells overexpressing Nlp (left panel, no induction of Nlp overexpression). B, localization of GCP2 in cells overexpressing the Nlp protein with or without treatment with GCP4 siRNA. C, microtubule regrowth assay on cells overexpressing the Nlp protein with or without expression of GCP2-mCherry. The graph shows the quantification of the number of microtubules after regrowth (35 cells scored per condition; error bars, mean ± S.D.). D, localization of GCP3-GFP (green) and GCP2-mCherry (red) in cells overexpressing Nlp. E, GFP lifetime distribution of GCP3-GFP with or without co-expression of GCP2-mCherry. F, GFP lifetime distribution of GCP3-GFP with or without co-expression of GCP3-mCherry. Histograms show the percentage of centrosomes according to the lifetime classes. Schemes on the right indicate the percentage of FRET efficiency.