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Binding affinities of sodium and potassium detected with RH421
Sodium or potassium titration curves in Fig. 5 were fitted to the Hill equation. The K0.5 and nH values represent averages from three experiments ±S.E.
| Isoform | K0.5Na+ (nH) | K0.5K+ (nH) |
|---|---|---|
| mm | mm | |
| α1β1 | 7.7 ± 0.5 (1.7 ± 0.13) | 0.6 ± 0.04 (1.8 ± 0.1) |
| α2β1 | 8.0 ± 0.3 (1.9 ± 0.2) | 1.5 ± 0.1 (1.5 ± 0.1) |
| α2β2 | 8.1 ± 0.3 (1.7 ± 0.2) | 5.1 ± 0.1 (1.6 ± 0.1) |
| α2β3 | 7.3 ± 0.6 (1.8 ± 0.1) | 4.8 ± 0.6 (1.5 ± 0.2) |
