FIGURE 4.

Three-dimensional structure of SCO6735 (magenta) superimposed on a human TARG1/C6orf130 (PDB code 4J5R, green) and bacterial, B. thetaiotaomicron (PDB code 2FG1, blue) proteins. Human protein is in complex with PARG inhibitor ADP-HPD. ADP-HPD, as well as amino acids important for ligand binding (Lys⁸⁴) and catalytic activity (Asp¹²⁵) and corresponding amino acids in bacterial structures (Gln⁸⁵ and Ala¹³⁰ in SCO6735 and Gln⁸² and Ala¹²⁸ in BT1257) are shown in stick representation. Amino acids that could not be well defined in the electron density maps are omitted and shown by dashed line.