FIGURE 4.

SDS-PAGE and quinoprotein staining of protein fractions. A, 10% gel stained for protein. B, electroblot stained for quinoproteins. A gel identical to that in A was subjected to electrophoretic transfer of the proteins and stained for quinoproteins as described under “Experimental Procedures”. The positions of migration of molecular mass markers in the stained gel are indicated. Lane 1, D547A GoxA as isolated in complex with GoxB. Lane 2, H466A GoxA as isolated in complex with GoxB. Lane 3, WT LodA. Lane 4, active WT GoxA; Lane 5, inactive WT GoxA in complex with GoxB. The molecular masses of the proteins of interest are: LodA, 82,905 kDa; GoxA, 76,285 kDa; and GoxB, 41,856 kDa.