FIGURE 7.

Cell death and apoptosis in AR42J cells transduced with control, CEL14R, or CEL MODY adenovirus. A, cell death was determined as described under “Experimental Procedures.” The level of cell death was significantly higher in cells expressing CEL MODY than in cells expressing CEL14R (p < 0.001) (n = 3). B, the amount of mRNA encoding ATF4 in each cell line was determined by qRT-PCR 48 h after transduction. The amount of ATF4 was significantly higher in cells expressing CEL MODY than in cells expressing CEL14R (p = 0.007) (n = 4). C, the amount of mRNA encoding CHOP in each cell line was determined by qRT-PCR 48 h after transduction. The amount of CHOP was significantly higher in cells expressing CEL MODY than in cells expressing CEL14R (p = 0.01) (n = 3). D, total cellular extracts from cells transduced with control, CEL14R, or CEL MODY adenovirus or treated with staurosporine were separated by SDS-PAGE, and cleaved PARP was detected by immunoblotting. A representative blot is shown. The amount of cleaved PARP relative to the mock sample is presented as a bar graph. The amount of cleaved PARP in the cells transfected with CEL MODY is significantly greater than the amount in the CEL14R-transfected cells (p < 0.001) (n = 5). E, activation of the NF-κB promoter was determined in a luciferase assay as described under “Experimental Procedures.” There was significantly more activation of the NF-κB promoter in cells transduced with adenovirus expressing CEL MODY than in cells transduced with CEL14R (p < 0.001) (n = 3). There was no difference among the mock, GFP, and CEL14R expressed proteins. Error bars represent S.D. **, p = 0.01; ***, p = 0.001.