FIGURE 2.

Stress induces a greater loss of all thymocyte subsets in mice lacking miR-205 in thymic epithelial cells. A, the percent of various thymocyte subsets following poly(I:C) treatment was determined by flow cytometry analysis staining with antibodies against CD4 and CD8 cell surface markers. B, the total thymic cellularity and the number of CD4⁺CD8⁺, CD4⁺CD8⁻ and CD4⁻CD8⁺ thymocytes were compared between untreated (day 0) and poly(I:C)-treated mice 2, 5, 8, and 11 days post-injection. C, the total thymic cellularity and subset cellularity in untreated and LPS-treated sibling controls and miR-205^fl/fl:Foxn1-Cre was calculated at 7 days post-injection. The effects of LPS on thymocyte subsets were determined by flow cytometric analysis of thymocytes stained with mAbs that detect the CD4 and CD8 cell surface markers. The thymii from the indicated mice were isolated 7 days post-LPS injection. *, p < 0.04; **, p < 0.006; ***, p < 0.0002; ****, p < 0.00001 (Student's t test). Data are representative of mean ± S.E. from at least 5 mice per group. All mice used for the poly(I:C) experiment were female. The LPS experiments were performed with male and female mice.