FIGURE 4.
The putative targets of miR-205 in TECs affect the expression of multiple chemokine regulated pathways. A, TECs were sorted from the thymii of 2 to 3 independent groups of control (lanes 1–3, 7, and 8) and miR-205fl/fl:Foxn1-Cre mice (lanes 4–6 and 9–11) from 8-week-old mice (lanes 1–6) or 5 days after a 2nd poly(I:C) treatment (lanes 7–11). RNA was isolated and used for gene expression comparisons. A heat map was used to indicate the up- (red) and down- (green) regulated genes from the indicated mice. B, Venn diagram demonstrating the number of up- and down-regulated genes that are common and unique to the steady state and stressed miR-205fl/fl:Foxn1-Cre TECs. C, Ingenuity Pathway Analysis was used to identify the most significant chemokine/chemokine receptor pathways differentially regulated in the absence of miR-205. D, Ingenuity Pathway Analysis of the genes increased (red) or decreased (green) coupled to the β-Catenin signaling pathway in miR-205-deficient TECs. Light green indicates genes uniquely down in stressed or 8 week TECs, whereas pink indicates genes uniquely up in 8 week or stressed TECs.