TABLE 2.
Disaccharide composition of C. elegans HS
Values are expressed as pmol of disaccharide/mg of dried worm homogenate. The means ± S.E. of three determinations is shown. The degree of sulfation is expressed as the average number of sulfate groups/disaccharide unit. ND, not detected (<0.01 pmol/mg).
| Composition | N2 | RB813 |
|---|---|---|
| pmol/mg (%) | ||
| ΔHexUAα1–4GlcNAc | 3.4 ± 0.41 (60) | 4.2 ± 0.37 (55) |
| ΔHexUAα1–4GlcNAc(6-O-sulfate) | 0.6 ± 0.01 (11) | 0.9 ± 0.09 (12) |
| ΔHexUAα1–4GlcN(2-N-sulfate) | 0.7 ± 0.01 (13) | 1.1 ± 0.24 (14) |
| ΔHexUAα1–4GlcN(2-N-,6-O-disulfate) | ND | ND |
| ΔHexUA(2-O-sulfate) α1–4GlcN(2-N-sulfate) | 0.8 ± 0.01 (14) | 1.3 ± 0.08 (17) |
| ΔHexUA(2-O-sulfate) α1–4GlcN(2-N-,6-O-disulfate) | 0.1 ± 0.01 (2) | 0.1 ± 0.01 (2) |
| Total disaccharide | 5.6 ± 0.35 | 7.6 ± 0.46 |
| Degree of sulfation | 0.57 | 0.64 |