Table 1.

Reagents and Materials for Culture Media, Cell Growth and Cryopreservation

#	Name	Company	Catalog #
1	Sodium Selenium	DIFCO	0608-15
2	Insulin	Sigma	1-5500
3	Human Transferrin	Sigma	T-2252
4	Putrescine	Sigma	P-7505
5	Sodium Bicarbonate	Fisher	S233-500
6	Sodium Selenite	Sigma	S5261
7	Bovine Serum Albumin	Sigma	A3156
8	B-27 Supplement	Invitrogen	17504-044
9	D-MEM/ F12 medium	GIBCO BRL	12100-046
10	D-MEM low glucose medium	GIBCO BRL	11054-020
11	Anti-PSA-NCAM IgM	Iowa DSHB	http://www.uiowa.edu/~dshbwww/
12	Recombinant Human EGF	Invitrogen	Cat.132476-051
13	Recombinant Human bFGF	Invitrogen	Cat.13256-029
14	Tris	Fisher	BP 152-1
15	D(+) galactose	Sigma	G0625
16	Kanamycin	Sigma	K0254
17	12.5, 75 cm2 T-Flasks	Falcon	353003
18	Petri dishes (Non-TC)	Falcon	351029
19	SYTOX	Invitrogen	S7020
20	Creatine	Sigma	C3630-100G
21	Tissue Culture Test tubes	Fisher	14-956-6A
22	5ml sterile pipettes	Falcon	357543
23	Cell strainers	BD Falcon	REF. 352350
24	12ml Syringes sterile	Tyco Healthcare	512852
25	20 ml Syringes sterile	Jone Kendall	520673
26	Cryo-Tube Vials	NUNC	366656
27	Cryogenic Freezing container	NALGENE	EW-44400-00
28	Neurobasal Medium	GIBCO	21103-049
29	B-27 Supp. without Vitamin A	GIBCO	12587-010
30	GlutaMAX	GIBCO	35050-061
31	Heparin	Sigma	H-3149
32	Normocin	InVivoGen	Ant-nr-1
33	Leukemia Inhibitory Factor	Millipore	LIF-1010
34	Fluorescent Fast Blue	Sigma	F5756
35	Progesterone	Sigma	P7556
36	Normocin
37	KnockOut DMEM	Gibco-BRL	10829-018
38	KnockOut serum replacement	GIBCO BRL	10828
39	Retinoic acid	Sigma	R2625
40	Accutase	Innov. Cell Tech.	AT104
41	Tissue Culture Flask, T115 w/ recloseable lid	TPP	TP90652
45	Anti-IgM whole Molecule