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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Curr Protoc Stem Cell Biol. 2016 Aug 17;38:2D.18.1–2D.18.27. doi: 10.1002/cpsc.11
Insulin 5 mg
Transferrin 50mg
Putrescine 16.1mg
Progesterone 20nM
Sodium Selenium 4µg 0.4mg/ml (4ml/litter of medium)
Sodium Bicarbonate 2.2g
Penicillin-Streptomycin 1 ml
Kanamycin 1 ml