Table 2. Details of Primers used for quantitative PCR.

The primers were based in the human cDNA sequences. The HUGO/GeneBank accession numbers, oligonucleotide sequences, position and amplicon size are outlined below and in S1 Table.

Isoform specificity of primers	Primer sequence	Position	GC content (%)	Predicted Melting Temperature (°C)	Product length
• PPP1R12ALZ+ • (NM_002480.2)	SENSE: 5′ TTGGAAATGGAAAAAAGGGAACG 3′	2936	39.1	76.8	120
	ANTISENSE: 5′ CCCATTTTCATCCTTTAGCCTCT 3′	3057	43.5
• PPP1R12ALZ- • (NM_002480.2+31 NT INSERT)	SENSE: 5’ TGTCGGCAAGAGTCAGTAT 3’	2957	50	85	104
	ANTISENSE: 5’ TGTCGGCAAGAGTCAGTAT 3’	3060	37.5
• PPP1R12A canonical • (NM_002480.2)	SENSE: 5’ AAGCACCACATCAACACCAA 3’	1704	45	60	192
	SENSE: 5’ ATGGTCACTGCCGTAGGAAC 3’	1895	55
• PPP1R12BLZ+ • (NM_002481.3)	SENSE: 5’ GAGATGGAGAAACGGGAGAG 3’	2797	55	78.9	129
	ANTISENSE: 5’ TCTGATGAGGGCACCATTTT 3’	2925	45
• PPP1R12BLZ- • (NM_002481.3+31 NT INSERT)	SENSE: 5’ TGTCCGGCAAGAGTCAGTAT 3’	2813	50	85	108
	ANTISENSE: 5’ GTGTCGGATTCAGTTCTGTTAA 3’	2919	37.5
• PPP1R12B canonical • (NM_002481.3)	SENSE: 5’ TGAGAAGCCCACAGACACTG 3’	2025	55	60	227
	ANTISENSE: 5’ ATAGCAGGTGGGAACTGGTG 3’	2251	55
• PPP1R12CLZ+ • (NM_017607.3)	SENSE: 5’ TGGAACTGGAGAGATTCGAGC 3’	2195	64	86.7	56.8
	ANTISENSE: 5’ TTGTCAGCGCGGAGGTC 3’	2288	52
• PPP1R12CLZ- • (NM_017607.3+31 NT INSERT)	SENSE: 5’ CGCAGAGGCAAGAACGCT 3’	2138	64	83.5	87
	ANTISENSE: 5’ CGCCCAGTAGATACTGACTCTT 3’	2239	52
• PPP1R16A • (NM_032902.5)	SENSE: 5’ TCTTCCCTCCCAGTGTTGTC 3’	669	55	91.5	200
	ANTISENSE: 5’ GTCACAGGCATTGATGTTGG 3’	862	50
• PPP1R16B • (NM_015568.2)	SENSE: 5’ CGGACAGGACCAACCTGTAT 3’	1244	55	86.4	198
	ANTISENSE: 5’ CTCGTGGGATCTTGGTAGGA 3’	1422	55
• MYH1 • (NM_005963)	SENSE: 5’AATCATAAGTGAAGAGTAATTTATCTAAC 3’	5896	24.1	56.4	95
	ANTISENSE: 5’ CATAAGTACAAAATGGAGTGACAAAG 3’	5990	34.6
• ACTA2 • (NM_005963)	SENSE: 5’ TGACGAAGCACAGAGCAAAA 3’	171	45	56.4	128
	ANTISENSE: 5’ GGGCAACACGAAGCTCATT 3’	298	56.5