Fig 5. Native conformations of the three individual components in AMSP-Fu₃₅.

(A) IFA was performed with late schizont stage parasites using AMSP-Fu₃₅ sera and co-localization was checked by co-immunostaining with sera against individual components (anti-PfMSP-1₁₉, PfAARP and PfMSP-3₁₁). The corresponding images stained with DAPI, which stains nuclear DNA; merged fluorescence images and bright field images are also shown. (B) Pearson’s correlation graphs for the co-immunostained slides, (i) AMSP-Fu₃₅ and PfAARP, (ii) AMSP-Fu₃₅ and PfMSP-3₁₁ and (iii) AMSP-Fu₃₅ and PfMSP-1₁₉, are shown and high correlation values showed co-localization. (C) Antibodies to AMSP-Fu₃₅ react with proteins corresponding to PfAARP, PfMSP-1₁₉ and PfMSP-3₁₁ in immunoblots of schizont stage parasite lysate from 3D7. Parallel immunoblots of the same lysate probed with anti-AMSP-Fu₃₅, PfAARP, PfMSP-3₁₁ and PfMSP-1₁₉ sera confirmed the proteins detected by the fusion sera.