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. 2016 Oct 31;11(10):e0164712. doi: 10.1371/journal.pone.0164712

Fig 6. Live-dead analysis indicates that E171 is not immediately cytotoxic to Caco-2 epithelia.

Fig 6

(a) Untreated Caco-2 display the traditional honeycomb arrangement of cells within the epithelium. Note the existence of no ethidium-positive (red) cells. The inset is a positive control for cell death. Red cells indicate cell death. (b) Exposure to 1 μg/mL of E171 (total fraction) after 24 hours. (c) Exposure to 1 μg/mL of submicron-enriched E171 after 24 hours. (d) Exposure to 1 μg/mL of submicron-enriched E171 after 24 hours. The scale bars are 20 μm.