Table 3. Mapping of T-lymphocyte epitopes on PRRSV proteins using overlapping peptide pools encompassing all open reading frames of PRRSVSD23983.
| Peptide pool | Gilt 1 PBMC | Gilt 2 PBMC | Gilt 3 PBMC | ||||||
|---|---|---|---|---|---|---|---|---|---|
| 30dpi | 44dpi | 58dpi | 33dpi | 44dpi | 58dpi | 33dpi | 44dpi | 58dpi | |
| Unstimulated: mean SFU (5 x SE) | 19 (15) | 60.5 (52.5) | 24.5 (2.5) | 37 (10) | 3 (10) | 4 (5) | 26.5 (22.5) | 6 (5) | 5.5 (7.5) |
| nsp1 | 129+* | 183+ | 141+ | 26 | 15 | 24+ | 29 | 18+ | 9 |
| nsp2-1 | 49+ | 92 | 56+ | 2 | 8 | 13+ | 13 | 3 | 1 |
| nsp2-2 | 133+ | 245+ | 179+ | 18 | 0 | 8 | 34 | 13+ | 9 |
| nsp2-3 | 31 | 45 | 30+ | 19 | 0.5 | 0 | 9 | 0 | 0 |
| nsp3,4 | 49+ | 72 | 47+ | 115+ | 3 | 3 | 20 | 1 | 0 |
| nsp5,6,7 | 76+ | 75 | 73+ | 26 | 6 | 11+ | 16 | 6 | 2 |
| nsp8,9 | 29 | 22 | 27 | 55+ | 4 | 8 | 11 | 5 | 3 |
| nsp9,10 | 35+ | 48 | 30+ | 10 | 1 | 4 | 14 | 1 | 0 |
| nsp10,11 | 20 | 44 | 21 | 9 | 1 | 3 | 23 | 0 | 0 |
| nsp12, 2TF | 20 | 18 | 20 | 4 | 0 | 2 | 11 | 9 | 0 |
| GP2a and b, ORF5a | 143+ | 141+ | 74+ | 17 | 7 | 27+ | 28 | 46+ | 11 |
| GP3, GP4 | 212+ | 226+ | 180+ | 59+ | 25+ | 47+ | 60+ | 74+ | 19+ |
| GP5, M, N | 333+ | >345+ | 308+ | 56+ | 30+ | 65+ | 88+ | 155+ | 80+ |
| M | 218+ | 180+ | 134+ | 26 | 35+ | 93+ | 61+ | 129+ | 55+ |
| E (GP2b) | 14 | 13 | 16 | 6 | 1 | 5 | 4 | 7 | 4 |
| GP3 | 42+ | 29 | 28+ | 27 | 17+ | 46+ | 17 | 8 | 3 |
| GP4 | 194+ | 163+ | 139+ | 38 | 24+ | 42+ | 33 | 65+ | 26+ |
| GP5 | 200+ | 263+ | 214+ | 23 | 13 | 25+ | 65+ | 75+ | 34+ |
| ORF5a | 11 | 19 | 14 | 25 | 1 | 0 | 22 | 6 | 2 |
| N | 22 | NA | NA | 3 | 4 | 7 | 7.5 | 14+ | 6 |
| Inactivated PRRSVSD23983 | 182+ | 204+ | 177+ | 27 | 0 | 3 | 51+ | 1 | 0 |
* Numbers are mean IFN-gamma ELISpot assay SFU/106 PBMC minus the mean of the appropriate unstimulated control; those adjusted SFU means that were greater than the unstimulated control SFU mean plus 5 times the SE were scored as positive and marked with a + in the table (for gilt 1 at 30 dpi those test SFU means >34 were positive; for gilt 1 at 44 dpi, >113; for gilt 1 at 58 dpi, >27 and so on). SFU means with > indicate that the SFU was higher than the upper count limit for ELISpot reader.