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. 2016 Oct 13;11(10):e0164723. doi: 10.1371/journal.pone.0164723

Table 1. Premier sequences used for amplification of EGFP and IL2 gene with PCR.

Primer name Primer sequences
EGFP F (NP/P) 5’-GGCGCGCCGGACTAGTGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACC-3’
R (NP/P) 5’-CTCTCAGGCCTTCTACCCGTATTTTTTCTTAATTACTTGTACAGCTCGTCCATGCC-3’
F (P/M) 5’-CCGCGGGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACC-3’
R (P/M) 5’-GTTTAAACCTTCTACCCGTATTTTTTCTTAATTACTTGTACAGCTCGTCCATGCC-3’
F (M/F) 5’-GGTTAACCACGGGTAGAAGATTGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACC-3’
R (M/F) 5’-CGACGCGTCGTTTTTTCTTACCTCATCTGTGTTTACTTGTACAGCTCGTCCATGCC-3’
F (F/HN) 5’-GGTTAACCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACC-3’
R (F/HN) 5’-CGCGTCGCAGTTACTTGTACAGCTCGTCCATGCC-3’
IL2 F (NP/P) 5’-GGCGCGCCCGGACTAGTGCCACCATGTACAGGATGCAACT-3’
R (NP/P) 5’-GGCCTAGAGGGCCTTCTACCCGTATTTTTTCTTAATCAAGTCAGTGTTGAGATGATG-3’
F (P/M) 5’-CCGCGGGGACCGCCACCATGTACAGGATGCAACT-3’
R (P/M) 5’-GTTTAAACCCTTCTACCCGTATTTTTTCTTAATCAAGTCAGTGTTGAGATGATG-3’
F (M/F) 5’-GTTAACCACGGGTAGAAGATTCCGCCACCATGTACAGGATGCAACT-3’
R (M/F) 5’-CGACGCGTCGTTTTTTCTTACCTCATCTGTGTTCAAGTCAGTGTTGAGATGATG-3’
F (F/HN) 5’-GGTTAACCGCCACCATGTACAGGATGCAACT-3’
R (F/HN) 5’-CGCGTCTCAAGTCAGTGTTGAGATGATG-3’