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. 2016 Nov 1;7:1708. doi: 10.3389/fmicb.2016.01708

FIGURE 2.

FIGURE 2

Influences of the linked Fc or Taishan Pinus massoniana pollen polysaccharide (TPPPS) on macrophage activity. Peritoneal macrophages were cultured with the various concentrations of ompA–Fc (2.5, 5, and 10 μg/mL), ompA (2.5, 5, and 10 μg/mL), TPPPS (12.5, 25, 50, and 100 μg/mL), and PBS for 24 h. The activity of peritoneal macrophages was determined by MTT assay (A,B). The neutral red uptake (C), NO (D), and TNF-α (E) production of peritoneal macrophages were detected as previously described. All values represent the means ± SD of triplicate experiments. Different lowercase letters above the columns indicate significant differences between the different groups (P < 0.05).