FIGURE 3.

The linked Fc and TPPPS adjuvant enhance phagocytosis of peritoneal macrophages. Initially, 5 μg/mL ompA–Fc mixed with 50 μg/mL TPPPS (A), 5 μg/mL ompA–Fc (B), 5 μg/mL ompA (C), and 50 μg/mL TPPPS (D) and 100 μL PBS (E) were separately added into 96-well cell culture plates, where fresh peritoneal macrophages were cultured. After 6 h incubation, IFA was used to analyze the ompA phagocytosis, and the rat anti-omp polyclonal antibody as primary antibody and FITC-conjugated mouse anti-rat IgG as secondary antibody were incubated successively.