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. 2016 Oct 1;6(10):2221–2234.

Figure 3.

Figure 3

Mfn2 is involved in PKM2-induced mitochondrial fusion. A: The mRNA and protein expression levels of Mfn2 are elevated in PKM2 overexpressed cells. MCF-7 cells were transfected with GFP, PKM1or PKM2 for 24 h, the expression of Mfn2 was evaluated by qPCR and western blot. **P < 0.01. B: The mRNA and protein expression levels of Mfn2 are decreased in PKM2 knockdown cells. MCF-7 cells were transfected with shcont, shPKM2-1 or shPKM2-2 for 24 h, the expression of Mfn2 was evaluated by qPCR and western blot. **P < 0.01. C: The expression of Mfn2 is decreased following Mfn2 siRNAs treatment. MCF-7 cells were co-transfected with GFP or GFP-PKM2 and Mfn2 siRNAs, then cells were harvested, the mRNA and protein expression levels of Mfn2 were analyzed by qPCR and western blot, respectively. **P < 0.01. D: The ATP production is increased in Mfn2 siRNA treatment cells. After MCF-7 cells were co-transfected with GFP or GFP-PKM2 and Mfn2 siRNAs, the ATP production was detected. *P < 0.05. E: The mtDNA copy number is decreased in Mfn2 siRNA treatment cells. After MCF-7 cells were co-transfected with GFP or GFP-PKM2 and Mfn2 siRNAs, the mtDNA copy number was detected. *P < 0.05.