Figure 4.

Knockdown of SEMA3F expression promotes the viability of osteosarcoma cells. A. The protein level of SEMA3F in SaOS and MG-63 cells after transfection of siRNAs against SEMA3F or scramble sequence was analyzed by Western blot. β-actin was used as the loading control. B. Growth curves of SaOS and MG-63 cells with transfected siRNAs or scramble sequence. Cell growth was determined by CCK-8. C, D. A colony-formation assay was performed to determine the oncogenic growth of SaOS and MG-63 cells transfected with siRNAs or scramble sequence. The data in the figure represent the averages ± SD of triplicate samples. *P<0.05 compared with the control group based on one-way ANOVA.