SEMA3F knockdown promotes EMT and activates Wnt/β-Catenin pathway in osteosarcoma cells. (A) Representative western blot images of E-cadherin, N-cadherin, β-catenin, GSK-3β, c-myc, MMP-7 and CD44 expression. The protein expression level of E-cadherin, N-cadherin, β-catenin, GSK-3β, c-myc, MMP-7 and CD44 in SaOS (B) and MG-63 (C) cells was determined. The data in the figure represent the averages ± SD of triplicate samples. *P<0.05 compared with the control group based on one-way ANOVA.