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. 2016 Oct 1;6(10):2299–2309.

Figure 3.

Figure 3

lncRNA-CRNDE negatively regulates miR-384 in human HCC. A. The qRT-PCR was used to detect the mRNA expression level of miR-384 in HCC tissues and paired adjacent normal tissues. The levels of transcript were normalized to U6 snRNA, (n = 87, ***P < 0.001). B. The correlation between lncRNA-CRNDE and miR-384 expression were measured (r = 0.546, P < 0.001). C. The mRNA expression level of miR-384 was measured by qRT-PCR in MHCC97H cells transfected with lncRNA-CRNDE or the control and in combination with miR-384 mock or mimics, (**P < 0.01, ***P < 0.001). D. The expression level of miR-384 was measured by qRT-PCR in HuH7 cells transfected with shRNA-CRNDE or the scramble and in combination with miR-384 mock or inhibitors, (**P < 0.01). E. The alleged binding sites of miR-384 on the lncRNA-CRNDE 3’UTR (Wt and Mut) were shown. The luciferase reporter assay was used to detect the activity of lncRNA-CRNDE in HEK 293T cells cotransfected with lncRNA-CRNDE (Wt or Mut) and miR-384 mimics or mock, (**P < 0.01, ***P < 0.001). F. The miR-384 mimics significantly decreased lncRNA-CRNDE-mediated cell proliferation. The proliferation ability was measured by MTT assays in MHCC97H cells transfected with lncRNA-CRNDE or the control, and then transfected with miR-384 mock or mimics, (***P < 0.001). G. The miR-384 inhibitors significantly promoted shRNA-CRNDE-mediated cell proliferation. The proliferation ability was measured by MTT assays in HuH7 cells transfected with shRNA-CRNDE or the scramble and then transfected with miR-384 mock or inhibitors (***P < 0.001).