Table 1.
Differentially upregulated (>1.20-fold) proteins identified by iTRAQ in F2 treated SGC7901 cells.
| Rank # | Accession | Gene symbol (GN) | Definition (description) | Score | Mass | Cov% | Ration | COG function-description |
|---|---|---|---|---|---|---|---|---|
| Up 1 | sp|P07305-2 | H1F0 | Isoform 2 of histone H1.0 | 51 | 35582 | 13 | 2.11 | — |
| Up 2 | sp|P20962 | PTMS | Parathymosin | 503 | 15782 | 23.5 | 1.32 | — |
| Up 3 | tr|B8ZWD1 | DBI | Diazepam binding inhibitor, splice form 1A(2) | 121 | 15706 | 28.9 | 1.31 | Acyl-CoA-binding protein |
| Up 4 | sp|Q16576 | RBBP7 | Histone-binding protein RBBP7 | 877 | 55737 | 24.5 | 1.25 | FOG: WD40 repeat |
| Up 5 | sp|P46779-2 | RPL28 | Isoform 2 of 60S ribosomal protein L28 | 524 | 22107 | 27.6 | 1.35 | — |
| Up 6 | tr|B2R514 | — | cDNA, FLJ92300, Homo sapiens COP9 subunit 6 (MOV34 homolog, 34 kD) (COPS6), mRNA | 74 | 39068 | 20.2 | 1.22 | Predicted metal-dependent protease of the PAD1/JAB1 superfamily |
| Up 7 | tr|B3KY12 | — | cDNA FLJ46581 fis, clone THYMU3043200, highly similar to splicing factor 3A subunit 3 | 527 | 71859 | 22 | 1.24 | Splicing factor 3a, subunit 3 |
| Up 8 | sp|Q71DI3 | HIST2H3A | Histone H3.2 | 617 | 19694 | 26.5 | 1.40 | Histones H3 and H4 |
| Up 9 | tr|Q9P0H9 | RER1 | RER1 protein | 118 | 28927 | 22 | 1.26 | Golgi protein involved in Golgi-to-ER retrieval |
| Up 10 | tr|A8K3Q9 | — | cDNA FLJ76611, highly similar to Homo sapiens ribosomal protein L14 (RPL14), mRNA | 781 | 35114 | 25.9 | 2.24 | Ribosomal protein L14E/L6E/L27E |
| Up 11 | sp|Q9Y3A2 | UTP11L | Probable U3 small nucleolar RNA-associated protein 11 | 94 | 44174 | 21.7 | 1.30 | Uncharacterized conserved protein |
| Up 12 | tr|F2Z388 | RPL35 | 60S ribosomal protein L35 | 99 | 15372 | 32.3 | 1.35 | Ribosomal protein L29 |
| Up 13 | sp|Q9NZZ3 | CHMP5 | Charged multivesicular body protein 5 | 268 | 32218 | 21 | 1.42 | — |
| Up 14 | tr|B2R4D8 | — | 60S ribosomal protein L27 | 398 | 23061 | 36 | 1.28 | Ribosomal protein L14E/L6E/L27E |
| Up 15 | tr|M0QXF7 | C19orf10 | UPF0556 protein C19orf10 (fragment) | 265 | 11851 | 25 | 1.24 | — |
| Up 16 | tr|D3DV26 | S100A10 | S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (P11)), isoform CRA_b (fragment) | 134 | 27935 | 8.3 | 1.21 | — |
| Up 17 | tr|H7C2N1 | PTMA | Thymosin alpha-1 (fragment) | 117 | 18283 | 8.8 | 1.30 | — |
| Up 18 | tr|G2XKQ0 | — | Sumo13 | 60 | 14938 | 11.9 | 1.22 | Ubiquitin-like protein (sentrin) |
| Up 19 | tr|I3L1Y9 | FLYWCH2 | FLYWCH family member 2 | 99 | 19302 | 47.2 | 1.45 | — |
| Up 20 | tr|M0R210 | RPS16 | 40S ribosomal protein S16 | 1105 | 19391 | 57.4 | 1.27 | Ribosomal protein S9 |
| Up 21 | sp|O43715 | TRIAP1 | TP53-regulated inhibitor of apoptosis 1 | 82 | 12050 | 18.4 | 1.36 | — |
| Up 22 | sp|P49207 | RPL34 | 60S ribosomal protein L34 | 187 | 18684 | 20.5 | 1.66 | Ribosomal protein L34E |
| Up 23 | sp|Q92522 | H1FX | Histone H1x | 342 | 35250 | 25.4 | 1.33 | — |
| Up 24 | tr|J3KRX5 | RPL17 | 60S ribosomal protein L17 (fragment) | 795 | 27382 | 38.5 | 1.26 | Ribosomal protein L22 |
| Up 25 | sp|P02795 | MT2A | Metallothionein-2 | 104 | 9915 | 52.5 | 1.42 | — |
| Up 26 | tr|Q6FIE5 | PHP14 | PHP14 protein | 72 | 17301 | 8.8 | 1.27 | — |
| Up 27 | tr|A0PJ62 | RPL14 | RPL14 protein (fragment) | 536 | 21409 | 43.5 | 2.85 | Ribosomal protein L14E/L6E/L27E |
| Up 28 | tr|G3XAA2 | MAP4K4 | Mitogen-activated protein kinase kinase kinase kinase 4 | 142 | 156989 | 2.7 | 1.24 | Serine/threonine protein kinase |
| Up 29 | tr|C9JNW5 | RPL24 | 60S ribosomal protein L24 | 666 | 24642 | 32 | 1.67 | Ribosomal protein L24E |
| Up 30 | sp|Q13951 | CBFB | Core-binding factor subunit beta | 197 | 24461 | 18.1 | 1.20 | — |
| Up 31 | tr|D3DUE6 | N-PAC | Cytokine-like nuclear factor n-pac, isoform CRA_c | 219 | 76728 | 14.5 | 1.24 | 3-Hydroxyisobutyrate dehydrogenase and related beta-hydroxy acid dehydrogenases |
| Up 32 | tr|K7EKW4 | ISOC2 | Isochorismatase domain-containing protein 2, mitochondrial (fragment) | 130 | 21202 | 17.4 | 1.34 | Amidases related to nicotinamidase |
| Up 33 | sp|Q9NQ55-2 | PPAN | Isoform 2 of Suppressor of SWI4 1 homolog | 73 | 63713 | 10.7 | 1.37 | — |
| Up 34 | tr|B3KMF8 | — | cDNA FLJ10869 fis, clone NT2RP4001677 | 127 | 12398 | 27.7 | 1.28 | — |
| Up 35 | sp|P62424 | RPL7A | 60S ribosomal protein L7a | 613 | 42316 | 27.1 | 1.78 | Ribosomal protein HS6-type (S12/L30/L7a) |
| Up 36 | tr|B4E0X1 | — | Beta-2-microglobulin | 185 | 17093 | 13.1 | 1.25 | — |
| Up 37 | tr|H0Y7A7 | CALM2 | Calmodulin (fragment) | 735 | 24209 | 30.5 | 1.26 | Ca2+-binding protein (EF-Hand superfamily) |
| Up 38 | tr|J3KTJ8 | RPL26 | 60S ribosomal protein L26 (fragment) | 363 | 15545 | 34 | 1.24 | Ribosomal protein L24 |
| Up 39 | tr|B4DJM5 | — | cDNA FLJ61294, highly similar to keratin, type I cytoskeletal 17 | 326 | 21291 | 24.9 | 1.46 | — |
| Up 40 | sp|Q9Y3C1 | NOP16 | Nucleolar protein 16 | 79 | 27925 | 20.8 | 1.24 | — |
| Up 41 | sp|Q16543 | CDC37 | Hsp90 cochaperone Cdc37 | 384 | 57730 | 29.6 | 1.22 | — |
| Up 42 | sp|P16401 | HIST1H1B | Histone H1.5 | 801 | 42644 | 17.3 | 2.38 | — |
| Up 43 | sp|Q07866-3 | KLC1 | Isoform G of kinesin light chain 1 | 642 | 81828 | 23.9 | 1.24 | FOG: TPR repeat |
| Up 44 | tr|B4DKJ4 | — | cDNA FLJ57738, highly similar to translationally controlled tumor protein | 344 | 19250 | 32.4 | 1.28 | — |