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. 2016 Jun 11;29(11):1576–1584. doi: 10.5713/ajas.16.0336

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Copyright © 2016 by Asian-Australasian Journal of Animal Sciences

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Protein purification of heterologously expressed NfAXE1 in Escherichia coli. (A) SDS-PAGE with Coomassie Blue staining of proteins at various stages of purification. M, Molecular markers (kDa). Lanes: 1, Cells harboring pET28a as control; 2, crude cells harboring pET28a-NfAXE1 after IPTG induction; 3, purified recombinant NfAXE1. (B) SP, Internal standard signal peaks; *, purified target protein peak analyzed using the microfluidic system. NfAXE1, Neocallimastix frontalis PMA02 acetyl xylan esterase; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.