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Rapamycin inhibits elongation of very-long-chain fatty acids 1 (ELOVL1) expression in a time-dependent manner. Cells were treated with 100 nM rapamycin for 8 h and 24 h, and ELOVL1 expression was detected by enzyme-linked immunosorbent assay (ELISA) (A) and Western blot (B). (C) The resolved bands of western blot were quantified using Gel-Pro Analyzer 4.0 (Media Cybernetics, Inc., Rockville, MD, USA). ELOVL1 was down regulated with increasing treatment times (** p<0.01).
