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Rapamycin induces a time-dependent decrease in the secretion of DHA in bovine mammary epithelial cells (BMECs). Cells were treated with 100 nM rapamycin for 8 h and 24 h, and DHA in medium was measured by enzyme-linked immunosorbent assay (ELISA). Rapamycin induced a time-dependent decrease in the secretion of DHA (* p<0.05).
