Figure 5.

Target species diffuse rapidly into the capture probe-derivatized, Parallume-encoded beads without agitation. Two bead types, which contain DNA capture probes homologous (red) or nonhomologous (green) toward a 60-nt dye-labeled target (a), are placed into a flow-through device (Figure 2h), imaged at 315 nm (a), and subsequently treated with an excess of 10 nM dye-labeled target without agitation. Images in panels (b) and (c) show the reporter signal of the same beads imaged with 532 nm excitation at ∼1 min (b) and 60 min (c) after target addition. The chart (bottom) shows the time course of the static target diffusion into and captured by the beads with the homologous capture probe.