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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Birth Defects Res A Clin Mol Teratol. 2016 Jul 20;106(10):803–813. doi: 10.1002/bdra.23543

Figure 3. In vitro effects of exogenous thyroid hormone on Twist 1 +/− cranial suture cell metabolism and differentiation.

Figure 3

A. Assessment of cell proliferation (MTS) for wild type control, Twist 1 +/− untreated, and Twist 1 +/− cranial suture cells treated with low (7.8ng/ml), medium (78ng/ml), and high (780ng/ml) doses of thyroxine for 3 and 7 days demonstrates no significant differences in metabolic activity between groups. B. Assessment of alkaline phosphatase activity (ALP) shows Twist 1 +/− cells to have slightly decreased ALP activity at 7 days, however, there were no significant differences between groups at either time point.