Fig. 1.

Schematic representation of sRNA position at M. hyopneumoniae 7448 genome and primers design. a Overview of the location of sRNA_35 in the M. hyopneumoniae 7448 genome region from 582,636 to 593,642 base pair. White arrows represent the genes position and the sRNA_35 is represented by a gray box. Representation from Artemis. b Primers location for the RT-PCR stem-loop reaction. Slim arrows indicate the amplicon orientation. Black portions represent amplification and gray portions represent the primers location. The amplification from specific forward primer to universal reverse primer is the entire sRNA length