Table 4. Pi-interactions between hesperetin and respective proteins.

The residues involved and length of each pi-interaction as well as the types of pi-interactions formed were analysed using the Discovery Studio 2.5.

Protein	Binding	Distance (Å)	Interaction
NsP1	Hesperetin:UNK1–ARG70:NE	5.2141	Pi-cation
	Hesperetin:UNK1–nsP1:TYR185	6.18796	Pi-pi
	Hesperetin:UNK1–nsP1:ARG252:NE	6.85892	Pi-cation
	Hesperetin:UNK1–ARG252:NE	6.0649	Pi-cation
	Hesperetin:UNK1–nsP1:PHE43	6.334	Pi-pi
	Hesperetin:UNK1–nsP1:LYS442:NZ	5.9124	Pi-cation
3TRK	Hesperetin:UNK1–3TRK:TYR1079	4.72556	Pi-pi
	Hesperetin:UNK1–3TRK:TYR1079	5.65846	Pi-pi
	Hesperetin:UNK1–3TRK:TYR1177	4.36635	Pi-pi
	Hesperetin:UNK1–3TRK:PHE1225:HB2	2.74925	Pi-sigma
3GPG	Hesperetin: UNK1–3GPG:TRP148	5.10212	Pi-pi
	Hesperetin: UNK1–3GPG:TRP148	4.15778	Pi-pi
	Hesperetin:UNK1–3GPG:LYS118:NZ	3.86706	Pi-cation
	Hesperetin:UNK1–3GPG:GLY30:HA1	2.84749	Pi-sigma
	Hesperetin:UNK1–3GPG:VAL113:HB	2.9202	Pi-sigma
	Hesperetin:UNK1–3GPG:TYR63	4.02708	Pi-pi
	3GPG:TRP148–Hesperetin:UNK1	4.22731	Pi-pi
	3GPG:TRP148–Hesperetin:UNK1	4.05885	Pi-pi
NsP4	Hesperetin:UNK1–nsP4:ARG374:NE	5.53545	Pi-cation
	Hesperetin:UNK1–nsP4:ARG70:NE	5.3946	Pi-cation
	Hesperetin:UNK1–nsP4:ARG70:NE	6.64674	Pi-cation
	Hesperetin:UNK1–nsP4:ARG70:NE	5.65215	Pi-cation
	Hesperetin:UNK1–nsP4:ARG70:NE	5.28131	Pi-cation
	Hesperetin:UNK1–nsP4:TYR22	4.15499	Pi-pi
	Hesperetin:UNK1–nsP4:ARG70:NE	4.49028	Pi-cation
	Hesperetin:UNK1–nsP4:TYR185	6.16947	Pi-pi
	Hesperetin:UNK1–nsP4:ARG70:NE	5.20374	Pi-cation
	Heesperetin:UNK1–nsP4:ARG70:NE	4.51522	Pi-cation
	Hesperetin:UNK1–nsP4:ARG70:HD1	2.61933	Pi-sigma
SPK2	Hesperetin:UNK1–SPK2:TYR372	4.91884	Pi-pi
	SPK2:TYR372–Hesperetin:UNK1	4.34443	Pi-pi
	Hesperetin:UNK1–SPK2:ARG351:NE	6.2923	Pi-cation