Table.
KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| InVivoMAb Anti-Mouse CTLA-4 | Bio X cell | Cat# BE0131; RRID: AB_10950184 |
| Anti-Human CD90 PE | eBiosciences | Cat# 12-0909-42; RRID:AB_10670624 |
| Anti-Human MCSP-1 PE | Miltenyi Biotec | Cat# 130-091-225; RRID:AB_244198 |
| Mouse Monoclonal Anti-Human IFNGR1 | Novus Biologicals | Cat# NB100-64793; RRID:AB_959548 |
| Anti-Mouse CD4 Pacific Blue | Biolegend | Cat# 100534; RRID:AB_493375 |
| Anti-Mouse CD8 BV785 | Biolegend | Cat# 100750; RRID:AB_2562610 |
| Anti-Mouse Foxp3-AF532 | eBiosciences | Cat# 58-5773-82 RRID:AB_11218870 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Collagenase Type I | Thermo Fisher Scientific | Cat# 17100017 |
| Collagenase D | Roche | Cat#11088866001 |
| DNase I | Thermo Fisher Scientific | Cat# EN0525 |
| Puromycin | Thermo Fisher Scientific | Cat# A1113803 |
| Insulin-Selenium-Transferrin Supplement | Thermo Fisher Scientific | Cat# 41400045 |
| Recombinant Human IFN-γ | Peprotech | Cat# 300-02 |
| Recominant Murine IFN-γ | Peprotech | Cat# 315-05 |
| SyBR Green PCR Master Mix | Thermo Fisher Scientific | Cat# 4309155 |
| Critical Commercial Assays | ||
| PowerPlex 16 HS System | Promega | Cat# DC21 |
| RNeasy Mini Kit | Qiagen | Cat# 74104 |
| RNeasy Plus Micro Kit | Qiagen | Cat# 74034 |
| miScript II RT Kit | Qiagen | Cat# 21816 |
| Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | Cat# 23227 |
| CellTiter 96 Aqueous One Solution Cell Proliferation Assay Kit | Promega | Cat# G3582 |
| CellTrace™ CFSE Cell Proliferation Kit | ThermoFisher Scientific | Cat# C34554 |
| Annexin V Apoptosis Detection Kit I | BD Biosciences | Cat# 556547 |
| GeneChip Human Genome U133 2.0 | Affymetrix | Part# 902483 |
| Deposited Data | ||
| Raw data files for microarray analysis | NCBI Gene Expression Omnibus | Accession# GSE85898 |
| Raw data files for WES | dbGap | Accession# pending |
| Experimental Models: Cell Lines | ||
| Human melanoma-derived cell lines (C1–6) | This paper | |
| GM-CSF-expressing B16/BL6 melanoma (GVAX) | James Allison | Quezada, S.A., et al. 2006 {Quezada, 2006 #14} |
| IFNGR1 shRNA-transduced B16/BL6 melanoma | This paper | |
| Scrambled shRNA-transduced B16/BL6 melanoma | This paper | |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J mice | Jackson Laboratories | Stock# 000664 |
| Recombinant DNA | ||
| Lentiviral particles encoding IFNGR1 shRNA | Santa Cruz | Cat# sc-35636-V |
| Lentivirial particles encoding scrambled shRNA | Santa Cruz | Cat# sc-108080 |
| Sequence-Based Reagents | ||
| SureSelect Human All Exon 44 Mb V2.0 Bait Set | Agilent | Part# 5190-2885 |
| SureSelect-XT Target Enrichment | Agilent | Part# 5190-2902 |
| Software and Algorithms | ||
| BWA Aligner (bwa-0.7.5a) | Li and Durbin, 2009 | http://bio-bwa.sourceforge.net |
| Picard (V1 112) “MarkDuplicates” module | Broad Institute | http://broadinstitute.github.io/picard |
| Mutect (v1.1.4) | Broad Institute | https://www.broadinstitute.org/cancer/cga/mutect |
| ExomeCN | Jianhua Zhang | MD Anderson Cancer Center |
| Affy | Gautier L, et al, 2004 | https://bioconductor.org/packages/release/bioc/html/affy.html |